Biochem 4-1

1.

1 point

The tail of mRNA in eukaryotes is catalyzed by guanylytransferase.

True

False

2.

1 point

If you wanted to isolate a specific protein, what type of gel electrophoresis would you perform?

Western Blot

Northern Blot

Southern Blot

Eastern Blot

3.

1 point

What amino acid is first for all proteins?

proline

methionine

stop codon

glycine

4.

1 point

Which of the following is NOT needed to initiate prokaryotic DNA replication?

Primase

DNA ligase

Single strand binding protein

DNA helicase

5.

1 point

Transcription converts DNA into RNA

True

False

6.

1 point

Genes in highly condensed chromatin are usually expressed.

True

False

7.

1 point

Which of the following genes acts constitutively in E. coli?

lac operon

glucose utilization enzymes

trp operon

8.

1 point

In wobble base pairing, inosine (I) can pair with which amino acids?

guanine (G)

thymine (T)

adenine (A)

cytosine (C)

uracil (U)

9.

1 point

Which of the following locations binds RNA polymerase in prokaryotes?

okazaki fragments

beta factor

TATA box

promoter

10.

1 point

Which of the following targets proteins to stay in the ER?

KDEL

SKL

mannose-6-phosphate

NLS

11.

1 point

Which of the following synthesizes nuclear mRNA in eukaryotes?

RNA polymerase I

RNA polymerase II

RNA polymerase III

Mitochondrial RNA polymerase

12.

1 point

Mutations of enzymes normally act in a recessive manner due to an excess of enzymes normally being produced by the body.

True

False

13.

1 point

Nonhomologous end-joining (NHEJ) is the safest way to repair double-strand DNA breaks.

True

False

14.

1 point

Which of the following is used in DNA fingerprints such as paternity tests?

variable number tandem repeats (VNTR)

transposons

ddNTPs

conserved DNA regions

15.

1 point

Deamination of cytosine is a common mutation. What would it yield?

adenine

guanine

hypoxanthine

uracil

16.

1 point

Which of the following are important for splicing of mRNA?

snRNA

rRNA

tRNA

siRNA

17.

1 point

A man is homozygous for a dominant mutation, and his wife is homozygous normal. What is the probability that their child will have the disease.

50%

100%

0%

25%

18.

1 point

Which of the following is an important aspect or use of chain terminators?

Western Blotting

Sanger DNA sequencing

differentiating between prokaryotic DNA gyrase and eukaryotic DNA topoisomerase

Cancer treatment by DNA synthesis inhibition

19.

1 point

What enzyme affects the potency of many different types of drugs?

cytochrome C

cytochrome P450

ubiquinone

rRNA 60S

20.

1 point

Which of the following targets newly synthesized proteins to peroxisomes?

amphipathic signal peptide

SKL

hydrophobic signal sequence

NLS

21.

1 point

Which of the following are used to make mRNA from DNA by RNA polymerase?

NTPs

dNTPs

NDPs

NMPs

22.

1 point

A father has an autosomal X-linked dominant mutation, and the mother is normal. What is the probability that their son has the mutation?

0%

50%

25%

100%

23.

1 point

What term refers to genetic disorders in which the disease process depends on whether the maternal or paternal gene is epigenetically silenced?

pleiotropy

heteroplasmy

genomic imprinting

X-linked disease

24.

1 point

What trinucleotide repeat expansion is seen in myotonic dystrophy?

GAA

CAG

CGG

CTG

25.

1 point

Which of the following is NOT the function of each DNA polymerase subunit?

DNA polymerase delta replicates mitochondrial DNA

DNA polymerase beta repairs DNA

DNA polymerase epsilon synthesizes DNA in the leading strand

DNA polymerase alpha makes primers for DNA replication

26.

1 point

Which of the following mutations is caused by UV light?

depurination

thymine dimers

deamination

intercalculation

27.

1 point

What enzyme is inhibited by Quinolones in order to treat bacterial infections?

DNA gyrase

DNA polymerase III

Ribonucleotide reductase (RNR)

Helicase

28.

1 point

Which of the following is important in recombinant DNA technology?

restriction endonuclease

reverse transcriptase

oligonucleotides

cDNA

29.

1 point

Which of the following rRNA sizes are made by prokaryotes?

23S

28S

16S

18S

5S

30.

1 point

Which of the following molecules are repaired by cells?

DNA

rRNA

tRNA

phospholipids

mRNA

31.

1 point

Which of the following is characteristic of transposons?

are not part of the human genome

require a separate protein encoding region for transposase

can cause spontaneous mutation

not involved in antibiotic resistance

32.

1 point

Which of the following is considered codominant?

Huntington's disease

Tay Sachs

hemophilia

blood types

33.

1 point

Which of the following binds to RNA polymerase II inhibiting it?

alpha-amantin

rotenone

cyanide

Dactinomycin

34.

1 point

Thymine dimers must be repaired by nucleotide excision repair (NER).

True

False

35.

1 point

For an individual that is heterozygous for a recessive disease, they would always show no manifestations of the disease.

True

False

36.

1 point

What enzymes prevents degradation of the ends of chromosomes?

telomerase

Primase

DNA ligase

DNA polymerase I

37.

1 point

What can DNA microarray technology be directly used for?

DNA amplification

presence of many different genes in a sample

recombinant DNA

present of many mutation on a single gene

38.

1 point

Rho independent termination require a GC-rich hairpin loop to terminate transcription.

True

False

39.

1 point

Which of the following can silence genes?

mRNA

rRNA

miRNA

snRNA

tRNA

40.

1 point

Heritable changed can be passed on to offspring through chromatin modifications such as methylation.

True

False

41.

1 point

Which of the following mutations would be most detrimental?

transversion

transition

missense

nonsense

42.

1 point

SNPs can cause VNTRs leading to RFLP that can be run on gels to differentiate between DNA samples.

True

False

43.

1 point

At what site of ribosomes do most tRNA initially bind?

T site

A site

E site

P site

44.

1 point

Prokaryotes use which of the following mainly in DNA replication?

RNA polymerase

DNA polymerase II

DNA polymerase I

DNA polymerase III

45.

1 point

How does mismatch repair know which strand is the correct one?

random

histone arrangement

tagged with different nucleotides

methylation

46.

1 point

Recessive mutations usually involve proteins that assemble into a polymeric structure.

True

False

47.

1 point

Which of the following is NOT a type of translocation mutation?

duplication

inversion

substitution

deletion

48.

1 point

Which of the following "antibiotics" inhibit eukaryotic cells?

streptomycin

tetracyclin

ricin

erythromycin

diphtheria toxin

49.

1 point

Which of the following is true of prokaryotic DNA replication?

uses Okazaki fragments on the lagging strand

semi-conservative

bidirectional

made in the 5' to 3' direction

two origins of repilcation

50.

1 point

Which of the following are NOT required for Sanger sequencing?

DNA topoisomerase

dNTPs

ddNTPs

Taq polymerase