MICRO BOC #247-278

1.

1 point

Which of the following genera include anaerobic gram-negative nonsporulating bacilli?

Brucella

Pasteurella

Actinomyces

Bacteroides

2.

1 point

A control strain of Clostridium should be used an anaerobe jar to assure:

that the indicator strip is checked

that an anaerobic enviorment is achieved

that plate media is working

that the jar is filled with a sufficient number of plates

3.

1 point

The major features by which molds are routinely identified are:

macroscopic characteristics and selective media

specialized sexual reproductive structures

biochemical reactions and microscopic morphology

macroscopic characteristics and microscopic morphology

4.

1 point

A sputum specimen from a patient with a known Klebsiella pneumoniae infection is received in the laboratory for fungus culture. The proper procedure for handling this specimen is to:

perform a direct PAS stain; if no fungal organisms are seen

incubate culture tubes at room temperature in order to inhinit the bacterial organism

include media that have cycloheximide and chloramphenicol added to inhibit bacterial organisms and saprophytic fungi

reject the current specimen and request a repeat culture when the bacterial organism is no longer present

5.

1 point

Many fungal infections are transmitted to man via inhalation of infectious structures. Which of the following is usually contracted in this manner?

Malassezia furfur

Histoplasma capsulatum

Sporothrix schenckii

Trichophyton rubrum

6.

1 point

A smear of skin tissue reveals fluorescent septate hyphae. The smear was prepared using:

calcofluor white

periodic acid-Schiff

acridine orange

auramine rhodamine

7.

1 point

An organism that demonstrates budding yeast cells with wide capsules in India ink preparation of spinal fluid is probably:

Cryptococcus neoformans

Blastomyces dermatitidis

Histoplasma capsulatum

Candida albicans

8.

1 point

The formation of germ tubes presumptively identifies:

Candida parapsilosis

Candida albicans

Candida tropicalis

Candida glabrata

9.

1 point

An HIV-positive patient began to show signs of meningitis. A spinal fluid was collected and cultured for bacteria and fungus. A budding, encapsulated yeast was recovered. Which organism is consistent with this information?

Cryptococcus neoformans

Microsporum audouinii

Aspergillus fumigatus

Sporothrix schenckii

10.

1 point

Gram stain examination of a CSF specimen indicates the presence of yeast-like cells with gram-positive granular inclusions. Which of the following techniques should be used next to assist in the identification of this organism?

periodic acid-Schiff

India ink

10% KOH

lactophenol cotton blue

11.

1 point

The recovery of some Cryptococcus species may be compromised if the isolation media contains:

chloramphenicol

gentamicin

penicillin

cycloheximide

12.

1 point

The most sensitive test for the initial diagnosis of cryptococcal disease is:

Gram stain

cryptococcal antigen

Giemsa stain

India ink