Biochem

1.

1 point

The biuret test does not work well with certain kinds of proteins, eg. with albumin (giving false negativity)

True

False

2.

1 point

The optimum T for trypsin is 40 ®C regardless of its source, mobility or stabilizers - other results must be erroneous

True

False

3.

1 point

Lactate, when administered in the form of infusion soln. (e.g. Ringer's lactate), can be used to treat metabolic acidosis

True

False

4.

1 point

Gallstones most frequently contain cholesterol and bilirubin salts

True

False

5.

1 point

For correct determination of glycated haemoglobin (i.e. to avoid interference) blood must be prevented from hamolysis

True

False

6.

1 point

Immunochemical methods may be utilised only in biochemical labs

True

False

7.

1 point

Trypsin is an exopeptidase hydrolysing peptide bonds of proteins and forming free-terminal and N-terminal amino acids

True

False

8.

1 point

To get serum, blood is centrifuged before coagulation, to get plasma it must be centrifuged after coagulation

True

False

9.

1 point

) Immunochemical methods generally have very high specificity, so they never require wash steps

True

False

10.

1 point

Mean, standard deviation (SD) and coefficient of variation (CV) may be used to describe "normally distributed" data only

True

False

11.

1 point

The oGTT test is not suitable for the follow up of diabetic patients as it represents a high glucose load

True

False

12.

1 point

To determine blood haemoglobin, care must be taken to prevent Hb from oxidation (anaerobic conditions are required)

True

False

13.

1 point

The time interval of the incubation of the substrate with serum in the samples and the respective blanks may be different

True

False

14.

1 point

Test for albuminuria should be performed together with the test for creatinine in the urine

True

False

15.

1 point

The kidney may compensate acid-base balance disturbances by e.g. hydrogencarbonate recovery

True

False

16.

1 point

Calculation of eGFR based on creatinine (e.g. MDRD equation) may be used in all subjects regardless of their age

True

False

17.

1 point

Stewart-Fenl concept of acid-base balance involves "hypochloremic alkalosis" and "hypoalbuminemi alkalosis"

True

False

18.

1 point

Friedewald formula involves TAG (and not just TC and HDL), because LDL contains a significant proportion of TAG

True

False

19.

1 point

Haemolytic jaundice is manifested by pale stools and dark urine containing little or no urobilinogen

True

False

20.

1 point

Urinary ketones determined by diagnostic strips are indispensable for making the diagnosis of diabetes mellitus

True

False

21.

1 point

The imunochemical test for blood in faeces (occult bleeding) may only be performed if blood is not visible in stools

True

False

22.

1 point

The first reaction involved in the routine determination of serum TAG is a redox reaction

True

False

23.

1 point

Acute respiratory acidosis is primarily compensated by 〖pCO〗_2 fall

True

False

24.

1 point

HDL concentration is routinely used as an important target of pharmacotherapy

True

False

25.

1 point

Elisa plates may contain Ab that are covalently bound to the bottom of the wells and specifically interact with antigen

True

False

26.

1 point

A combination of pH 7.0, 〖pCO〗_25.3 kPa and BE +10mmol/l may frequently be encountered in the ICU unit

True

False

27.

1 point

If creatinine clearance is normal, both biochem parameters (serum and urine creatinine) must also be within normal range

True

False

28.

1 point

Absolute values of immunoassays are often incomparable among different labs using different methods

True

False

29.

1 point

Lipid profile cannot be used alone to assess global cardiovascular risk (SCORE table)

True

False

30.

1 point

Alpha-amylase cleaves all alpha-glycosidic bonds in saccharides

True

False

31.

1 point

Michaelisconstant (Km) determines the affinity of enzyme to its substrate

True

False

32.

1 point

Double reciprocal plot by lineweaver and Burk is useful since it enables graphic determination of Vmax

True

False

33.

1 point

Feasting and alcohol consumption 2 h before phlebotomy invalidate the determination of baseline plasma TAG

True

False

34.

1 point

Most errors in the clinical laboratory testing process result from unprecise pipetting or undesired bubbles in the tubing

True

False

35.

1 point

Knowledge of the substrates dilution in the incubation mixture is not necessary for correct determination of Km

True

False

36.

1 point

The secretion of creatinine in the renal tubules may lead to overestimation eGFR (calculated from serum creatinine)

True

False

37.

1 point

The determination of Cystatin C is always preferred before creatinine due to its cheaper analysis by turbidimetry

True

False

38.

1 point

According to Lambert- Beer law V0 is proportional to the rate of change of the solutions absorbance

True

False

39.

1 point

Immunoassays may be used to determine the conc of Ag as well as Ab

True

False

40.

1 point

In analytical terms, true but unprecise analytical process is more "fit for purpose" than its untrue but precise counterpart

True

False

41.

1 point

"Direct" bilirubin is determined directly, i.e. reacts with a specific antibody without the need of accelerators

True

False

42.

1 point

A process is considered "precise" if it associated with random error of low magnitude, regardless of "trueness"

True

False

43.

1 point

Glycated haemoglobin is abbreviated as HbA1c

True

False

44.

1 point

Capillary glucose examined by personal glucometer may be used by a patient to make a diagnosis of diabetes mellitus

True

False

45.

1 point

In order to gain reducing properties, sucrose solution must be exposed to the action of sucrase

True

False